CLCN7 Knockout cell line (U-2 OS)

CLCN7 Knockout cell line (U-2 OS) Order

Catalog#

YKO-H856

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

CLCN7 Knockout cell line (U-2 OS)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:5

Species

Human

Gene Name

CLCN7

Gene ID

1186

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% McCOY's 5A+10%FBS

Target region

Knockout region: Exon 1 (based on transcript CLCN7-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:U-2 OS			STR Info (Cell bank) Cell Line:U-2 OS
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	13	13		13	13
D7S820	11	12		11	12
D16S539	11	12		11	12
VWA	14	18		14	18
TH01	6	9.3		6	9.3
AMEL	X	X		X	X
TPOX	11	12		11	12
CSF1PO	13	13		13	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CLCN7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-2 OS cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CLCN7 gene knockout CLCN7 Knockout cell line (U-2 OS) will be expanded and cryopreserved.

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